Regarding how separable the proteins will be, it depends on how they may interact at high concentrations. Clearly, the deformability required for the formation of the Early protein 2 C-terminal DNA-binding domain-DNA complexes of various types is based not only on the rigidity of the base sequences in the DNA spacers, but also on the intrinsic deformability properties of each domain. Conformation specific antibodies locate endogenous oligomeric E7 species in the cytosol of 3 model cell lines, strongly co-localizing with amyloid structures and dimeric E7 localizes to the nucleus. Thanks Paul Riggs, anyway, it is our fault as a comunity of lazy biochemists who expect everything sorted out in manuals. E7N domain alignment without gaps. Gluthationylation of Cys 24 abolishes Rb binding, which is reversibly recovered upon reduction.
Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7.
If you know the expected molecular weight, run a well calibrated size exclusion chromatography S75 or S depending on the size.